DNA

Part:BBa_K1667004:Design

Designed by: Jinyan Chu   Group: iGEM15_Shiyan_SY_China   (2015-09-07)


suicide gene ccdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 255


Design Notes

There is a rbs sequence in front of the ccdB gene fragment to ensure the translation of it.


Source

The sequence of the ccdB gene in this part is from BB_K145151 and BB_K1010007 in igem.

References